Details:
LymphoSpinMediumisareadytouse,steriledensitygrADIentmediumfortheisolationofmononuclearcells(PBMC)inhighyieldfromfreshperipheralwholebloodandbuffycoatbyasimplecentrifugationprocedure.Mononuclearcellscanbeusedforawiderangeofdownstreamapplicationssuchasmagneticactivatedcellsorting(suchasMACS1)orstemcellresearch.
LymphoSpinMediumcanbeusedassubstituteforFicoll™,2andLymphoprep™,3withoutanyneedtochangetheexistingprotocols.
SeparationschemeforPBMCenrichmentwithPBMCSpinMedium
AtfirstcarefullylayerthesamplematerialontopofthePBMCSpinMedium.Avoidamixingofthetwophases.Alternativelyit’spossIBLetousepluriMatetubes.Thebarrierpreventsthemixingofthetwophasesandenablestopourofftheenrichedcellsafterthefirstcentrifugationstep.Afterthedensitycentrifugationaspiratetheupperlayer(Plasmaanddilutionbuffer).Afterwardstransferthemononuclearcelllayertoanewconicaltubeandwashthecells.
Fig.1:PreparationofPBMCwithLymphoSpinMedium.Thesamplematerialwillbeoverlayedontopofdensitygradientmedium.
Fig.2.:Layersafterdensitygradientcentrifugation.ThePBMCcanbefoundontopoftheLymphoSpinMedium.Erythrocytes,Granulocytesanddeadcellswillpassthemediumandcanbefoundatthebottomofthetube.
1MACSisatrademarkofMiltenyiBiotecGmbH;2Ficoll™isatrademarkofGEHealthcare;3Lymphoprep™isatrademarkofAxis-Shield/Alere
MoreInformation:
SampleMaterial | WholeBlood,PBMC,BuffyCoat,CordBlood,BoneMarrow,PrimaryCellSolution |
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StorageCondition | RoomTemperature |
RegulatoryStatement | Forresearchuseonly.Notforuseindiagnosticprocedures, |