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Leuko Spin Medium is a ready to use, sterile density gradient medium for the isolation of Leukocytes in high yield from fresh peripheral blood and buffy coat by a simple centrifugation procedure.

Mononuclear and polymorphonuclear (multi-lobed nuclei) cells can be enriched by a simple density gradient centrifugation with Leuko Spin Medium. The enriched Leukocytes can be used for a wide range of downstream applications such as magnetic activated cell sorting (MACS¹) or stem cell research.

Separation scheme for Leukocyte enrichment with Leuko Spin Medium

At first carefully layer the sample material on top of the Leuko Spin Medium. Avoid a mixing of the two phases. Alternatively it’s possible to use pluriMate tubes. The barrier prevents the mixing of the two phases and enables to pour off the enriched cells after the first centrifugation step. After the density centrifugation step aspirate the upper layer (Plasma and dilution buffer). Then transfer the mononuclear and the polymorphonuclear cell layer to a new conical tube and wash the cells.

Fig. 1: Isolation of all Leukocytes with Leuko Spin density separation medium.

Fig. 2: Enriched peripheral blood mononuclear and polymorphonuclear cells.

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